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Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Dasgupta, T.P; Aquart, D.V
Author Affiliation, Ana.
n/a
Article Title
Transfer of nitric oxide from nitrovasodilators to free thiols- : Evidence of two distinct stages
Medium Designator
n/a
Connective Phrase
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Journal Title
Biochemical and Biophysical Research Communications
Translated Title
n/a
Reprint Status
Refereed
Date of Publication
2005
Volume ID
335
Issue ID
3
Page(s)
730-733
Language
n/a
Connective Phrase
n/a
Location/URL
n/a
ISSN
n/a
Notes
n/a
Abstract
Two distinct stages, which can be monitored spectrophotometrically, have been observed for the first time in multiple reactions between thiol, such as l-cysteine and some well-known nitrovasodilators, namely S-nitroso-N-acetyl-d,l-penicillamine (SNAP), S-nitrosoglutathione (GSNO), and S-nitrosocaptopril (SNOCap) in aqueous solution (in the presence of EDTA). The first part of the reaction occurs at stopped-flow time scale ( approximately 10(-2)s(-1)) in one single step and has been found to be transnitrosation; followed by a slow decomposition of the products of the transnitrosation reaction with the formation of a variety of nitrogen products. Reactivity with regard to the first stage occurs in the order GSNO>SNAP>SNOCap. The second stage shows first-order rate constants of the order 10(-4)s(-1), although some degree of complexity exists in elucidating an accurate rate equation with which a comparative study could be done.....
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