Cadrin, M.; McFarlane-Anderson,Norma; Harper, M.E.; Gaffield, J; Begin-Heick, N.
Author Affiliation, Ana.
Tropical Metabolism Research Unit
Comparison of the subcellular distribution of G-proteins in hepatocytes in situ and in primary cultures
Journal of Cell Biochemistry
Date of Publication
Compares the subcellular localization of the heterotrimeric G-proteins in hepatocytes in situ to that in hepatocytes in primary culture. Also investigates the ability of various ligands to activate adenylyl cyclase (AC) in membrane preparations. In hepatocytes in situ the G proteins were mainly localized at the plasma membrane while in hepatocytes in culture they were predominantly cytoplasmic. The localization of the G-proteins in hepatocytes in situ correlates with their role in signal transduction. In homogenates prepared from the cultured cells, ligands which stimulate AC via Gs alpha were without effect, which was consistent with the localization of Gs alpha in the cytoplasmic and nuclear compartments. The 'relocalization' of the G proteins to the cytoplasm when cells are cultured suggests that transmembrane signalling may be regulated by cell differentiation and cell-cell and cell-extracellular matrix interactions....