View
Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Asemota, Helen N.; Ramser, J.; Lopez-Peralta, C.; Weising, K.; Kahl, G.
Author Affiliation, Ana.
Biotechnology Centre
Article Title
Genetic variation and cultivar identification of Jamaican yam germplasm by random amplified polymorphic DNA analysis
Medium Designator
n/a
Connective Phrase
n/a
Journal Title
Euphytica
Translated Title
n/a
Reprint Status
n/a
Date of Publication
1996
Volume ID
92
Issue ID
n/a
Page(s)
341-351
Language
n/a
Connective Phrase
n/a
Location/URL
n/a
ISSN
n/a
Notes
n/a
Abstract
Using random amplified polymorphic DNA (RAPD) analysis to characterise eleven cultivars of the five economically most important yam species grown in Jamaica (Dioscorea alata, D. cayenensis, D. rotundata, D. trifida and D. esculenta). Amplification of genomic DNA samples with nine different arbitrary 10mer primers revealed a total of 338 different band positions, ranging in size from 0.3 to 2.5 kb. RAPD patterns proved to be highly reproducible and somatically stable. While no variation was observed among plants belonging to the same cultivar, a large number of intervarietal and interspecific polymorphisms enabled us to reliably discriminate between all Jamaican cultivars investigated.....
read more
Keywords
n/a