Mitchell, Sylvia A.; Asemota, Helen N.; Ahmad, Mohammed, H
Author Affiliation, Ana.
Factors affecting the in-vitro establishment of Jamaican yams (Dioscorea spp.) from nodal pieces
Journal of the Science of Food and Agriculture
Date of Publication
Examines factors such as inorganic ammonium, growth regulator supplements, propagule type and subculture period affecting the in-vitro establishment of Dioscorea cayenensis and D trifida. The results indicate that for D cayenensis, transfer of whole plantlets to fresh initiation media prior to cutting into nodal segments and transfer to establishment media was beneficial. Addition of 800 mg litre-1 NH4NO3 to basal medium (BM) containing 0.5 mg litre-1 benzylamino purine (BAP) caused a significant improvement in nodal numbers and resulted in 11.8 propagules per original explant after 8 weeks or 184.1 pro-pagules after 16 weeks in-vitro. Rapid establishment of D trifida cv Short Neck Yampie depended on the original explant source. Explants obtained from small tuber vines grew faster on establishment than those obtained from leafy cuttings. Initiation on BM supplemented with 0.5 mg litre-1 kinetin (BM0.5K) and establishment on BM supplemented with 0.5 mg litre-1 BAP (BM0.5BAP) produced the optimum result for the vines obtained from small tubers (24.0 propagules after 8 weeks of culture). For explants obtained from leafy cuttings, initiation on BM0.5BAP and establishment on BM0.5K gave optimum results (15.8). Quick establishment of initiated plantlets shown by increased shoot response and high multiplication rates were achieved in this study.....