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Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Muzac-Tucker, I.; Ahmad, Mohammed, H
Author Affiliation, Ana.
Biotechnology Centre
Article Title
Rapid detection of polymorphism in yams (Dioscorea sp.) through amplification by polymerase chain reaction and rDNA variation
Medium Designator
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Connective Phrase
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Journal Title
Journal of the Science of Food and Agriculture
Translated Title
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Reprint Status
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Date of Publication
1995
Volume ID
67
Issue ID
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Page(s)
303-307
Language
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Connective Phrase
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Location/URL
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ISSN
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Notes
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Abstract
Ribosomal DNA (rDNA) from rice (Oriza Sativa) was used to examine polymorphism in 12 yam (Dioscorea sp) cultivars. Restriction enzyme digests of total yam DNA was probed with the pRR217 probe containing the entire repeat unit of rDNA from rice. The polymerase chain reaction was also used to amplify genomic DNA of six of the 12 cultivars studied using random primers. The amplification patterns of D rotundata-cayenesis cv 'tau' suggested that 'tau' is more closely related to D rotundata sp than it is to the D cayenesis sp. The results showed polymorphisms among the different yam cultivars.....
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Keywords
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