Asemota, Helen N.; Osagie, A.U.; Kahl, G.; Gorz, A.
Author Affiliation, Ana.
A simple and efficient protocol for the isolation of yam (Dioscorea specie) RNA
Nigerian Journal of Biochemistry and Molecular Biology
Date of Publication
Polysaccharides, protein, secondary metabolites and mucilaginous secretions interfere with the extraction of high quality RNA from yam, Dioscorea specie. A method for RNA extraction is described which overcomes these problems. Repeated organic solvent extraction with phenol/chloroform/amyl alcohol (25:24:1) improved the RNA purity. The method is appropriate for simultaneous processing of many samples and it can be scaled up easily for bulk preparation.....