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Publication Type
Journal Article
Author, Analytic
Asemota, Helen N.; Osagie, A.U.; Kahl, G.; Gorz, A.
Author Affiliation, Ana.
Biotechnology Centre
Article Title
A simple and efficient protocol for the isolation of yam (Dioscorea specie) RNA
Medium Designator
n/a
Connective Phrase
n/a
Journal Title
Nigerian Journal of Biochemistry and Molecular Biology
Translated Title
n/a
Reprint Status
Refereed
Date of Publication
1999
Volume ID
14
Issue ID
n/a
Page(s)
63-69
Language
n/a
Connective Phrase
n/a
Location/URL
n/a
ISSN
n/a
Notes
n/a
Abstract
Polysaccharides, protein, secondary metabolites and mucilaginous secretions interfere with the extraction of high quality RNA from yam, Dioscorea specie. A method for RNA extraction is described which overcomes these problems. Repeated organic solvent extraction with phenol/chloroform/amyl alcohol (25:24:1) improved the RNA purity. The method is appropriate for simultaneous processing of many samples and it can be scaled up easily for bulk preparation.....
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