Chin, Melanie; Morris, Walter; Tennant, Paula F.; Ahmad, Mohammed H.; Gonsalves, Dennis
Author Affiliation, Ana.
Department of Life Sciences
Biological and molecular characterization of papaya ringspot virus isolated from papaya orchards of different locations in Jamaica
Jamaican journal of science and technology
Date of Publication
Papaya ringspot virus type-p (PRSV) may have been present in Jamaica as early as 1929. However, it has only recently become a serious threat to papaya production in the island. Both newly established commercial orchards and traditional papaya growing regions of the island are plagued with PRSV. In this study the diversity of PRSV from four locations of the major papaya producing areas in Jamaica was examined to assist in the development of transgenic papaya for the control of the disease across the island. Serology and host range confirmed that the virus samples collected were type-p. Moreover, the host range examined showed no differences in pathogenicity either in symptom expression or in the rate of symptom development. Reverse transcriptase-polymerase chain reaction was used to amplify the coat protein genes of the virus samples. Comparative restriction fragment length polymorphism (RFLP) analyses did not differentiate between samples from the four locations; all gave similar RFLP patterns suggesting low variability within a location and between the four locations examined. The coat protein gene of one of the samples was sequenced. The Jamaican isolate shares 90.2 to 94% amino acid similarities with type-p strains from the Caribbean, the Americas, and Australia. Other isolates are to be cloned and similarly sequenced, however, it appears that transgenic papaya resistant to Jamaican PRSV isolates should be obtained using viral coat protein genes.....