Brown, V.M.D.; Asemota, Helen N.; Thangavelu, M.; Mantel, S.H.
Author Affiliation, Ana.
Department of Basic Medical Sciences
Genetic variation among dasheen (colocasia sp.) and cocoyam (xanthosoma sp.) grown in Jamaica by PCR based analyses
Jamaican journal of science and technology
Date of Publication
Using 17 accessions of cocoyam and 18 accessions of dasheen collected randomly from 14 farms in 8 parishes of Jamaica, PCR-based analyses were carried out to assess the genetic diversity within and between the species, and to develop markers linked to important traits useful for the assessment and control of bacterial infections. RAPD analyses using selected random 10-mer primers and the 3' -anchored microsatellite primer, -(CA)8RY-revealed groups of accessions that shared a similar and highly diverged amplification profiles in the samples used. PCR-based analyses of mitochondrial sequence using NAD 4.2 -NAD 4.3 and rps14 - COB and chloroplast sequence using trnL 03 - trnL 04 and trnR 01 - trnQ 01 revealed polymorphism within plastid and mitochondrial genomes of Xanthosoma sp. and Colocasia sp. The absence of NAD 4.2 - NAD 4.3 amplified fragment for Colocasia sp. indicates that it can be used to differentiate between species of Xanthosoma and Colocasia. Restriction analysis of amplified fragments provides evidence that at least two accessions of Xanthosoma sp and Colocasia sp. possess two trnL sequences. This is unusual because plants usually only posses a single trnL sequence. Analyses revealed that there are at least four (4) varieties of Xanthosoma and three (3) varieties of Colocasia in Jamaica.....