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Publication Type
Conference Proceedings
UWI Author(s)
Author, Analytic
McKoy, Marsha-Lyn G.; Simon, Oswald R.
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Author Affiliation
Department of Basic Medical Sciences
Paper/Section Title
Cyclic GMP - independent effects of nitric oxide in the pregnant rat uterus [Abstract]
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Proceedings Title
XIVth World Congress of Pharmacology: Meeting Abstracts
Date of Meeting
July 7-12, 2002
Place of Meeting
San Francisco, CA
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Date of Publication
2002
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Abstract
A proposed mechanism of inhibition of myometrial contractions by NO is the activation of guanylate cyclase, leading to increased intracellular levels of cyclic GMP (Yallampalli et al., 1993 & 1994; Izumi et al., 1993). However, evidence has also been presented against the operation of this system in the uterus (Kuenzli et al., 1996 & 1998; Bradley et al., 1998). The present study seeks to determine whether NO may be involved in the modulation of uterine contractions in rats, and whether this effect is dependent on guanylate cyclase activation. Uterine strips obtained from pregnant rats were exposed to several agents which alter tissue levels of NO and cyclic GMP, and the effects of these agents on spontaneous and agonist-evoked contractions were determined by monitoring isometric tension developed in the muscle strips. Although NO and cyclic GMP attenuated spontaneous and agonist-evoked uterine contractions, blockade of guanylate cyclase did not inhibit the ability of NO to produce relaxation. These findings are supported by previous reports from this lab (McKoy & Simon, 2001) on investigations involving the effects of SIN-1 on cyclic GMP levels and contractions in the pregnant mouse uterus. Results suggest that NO attenuates uterine contractions in rats by a mechanism that is independent of guanylate cylase activation and increases in tissue levels of cyclic GMP.....
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