Barrett, K.; McGrowder, Donovan A.; Brown, Paul D.; Ragoobirsingh, Dalip
Author Affiliation, Ana.
Department of Basic Medical Sciences
Nitric Oxide Inhibits Insulin-Stimulated Glucose Transport in Adipocytes [Abstract]
West Indian Medical Journal
Date of Publication
53 Suppl. 2
Objective: To investigate the role of nitric oxide (NO) in the pathogenesis of Tyoe 2 diabetes mellitus by measuring the uptake of D-glucose -[ 1-3H(N) ] in the absence or presence of insulin in adipocytes from rats treated with the NO-releasing drug, streptozotocin. Design and Methods: Eight adult Wistar rats per test group, weighing 120-300 g were injected with streptozotocin and nicotinamide to induce Type 2 diabetes mellitus. Control rats were injected with saline. After four weeks, an oral glucose tolerance test was performed. Control and diabetic rats were fastened overnight then sacrificed. Blood was collected for insulin determination and D-glucose-[ 1-3H(N) ] uptake into adipocytes in the absence and presence of insulin. Conclusion: The NO-releasing drug has a direct inhibitory effect on glucose uptake in adipose tissue and may thereby have a role in the aetiology of Type 2 diabetes mellitus.....