Obiefuna, Idongesit; Young, Ronald E.
Author Affiliation, Ana.
Department of Basic Medical Sciences
Concurrent Administration of Neem Extract Prevents Development of Hypertension and Accompanying Alterations in the Electrocardiogram Patterns in Deoxycorticosterone Asetate Salt Hypertensive Rats
West Indian Medical Journal
Date of Publication
52 Suppl. 6
Objective: We investigated the chronic effect of neem-leaf extract on the developmetn of hypertension ans on the electrocardiogram (ECG) in deoxycorticosterone acetate (DOCA) salt treated animals. Method: Over five to six weeks, inbred male Wistar rats with a starting weight of 190 g were given either: (1) twice weekly subcutaneous (sc) injections of vehicle (soybean oil, 0.25 ml per animal) for the first two weeks, plus normal drinking water 9controls); (2) twice weekly (sc) injections (weeks 1 and 2) only of 15 mg/kg DOCA dissolved in vehicle, plus drinking water containing 1.0% NaCl and 0.03% KCI (DOCA - salt group); (3) 20 mg/kg of aqueous neem extract daily, in addition to the DOCA-salt treatmen (DOCA-salt-neem group). All groups (8-12 animals) received normal rat pellets ad libitum and their blood pressure was measured weekly. Terminally, the animals were anaesthetized and ECG's recorded using sc pins in a Lead II configuration.....