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Publication Type
Journal Article
Author, Analytic
Saunders, Geoffrey; Roye-Green, Karen J.; Bodonaik, Nabin C.; Smikle, Monica
Author Affiliation, Ana.
Department of Microbiology
Article Title
Plasmid content of Extended Spectrum Betalactamase (ESBL) producing Eschericha coli [Abstract]
Medium Designator
n/a
Connective Phrase
n/a
Journal Title
West Indian Medical Journal
Translated Title
n/a
Reprint Status
Refereed
Date of Publication
2002
Volume ID
51 Suppl. 4
Issue ID
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Page(s)
31
Language
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Connective Phrase
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Location/URL
n/a
ISSN
0043-3144
Notes
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Abstract
Objective: To identify E Coli producing extended-spectrum beta-lactamase (ESBL) and examine plasmid content asssociated with this resistance mechanism.Method: Clinical laboratory isolate from University Hospital of the West Indies patients were examined for resistance to the third generation cephalosporins: ceftazidime and cefotaxime. A double-disc diffusion test was used to confirm the presence of clavulanate-inhibitable ESBLs. Isolates were grown to 20 ml volume in selective broth for plasmid extraction using PlasmidPURE midipreps. DNA obtained was electrophoresed at 12 V/cm through 1.0% agarose in TBE buffer for up to 8 hours and then stained wih ethidium bromide.Conclusion: High molecular weight plasmids conferring ESBL production E coli are expected to have transferred from resident ESBL klebsiella. The result obained is consistent with this theory, butmore sophisticated analysis is required for proof.....
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