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Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Lorenzo-Morales, J.; Barrett, D. M.; Ortega-Rivas, A.; Valladares, B.; Lindo, John F.
Author Affiliation, Ana.
Department of Microbiology
Article Title
Isolation and Identification of Pathogenic Acanthamoeba Strains from Water Sources in Jamaica, West Indies [Abstract]
Medium Designator
n/a
Connective Phrase
n/a
Journal Title
West Indian Medical Journal
Translated Title
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Reprint Status
Refereed
Date of Publication
2004
Volume ID
53 Suppl. 5
Issue ID
n/a
Page(s)
19
Language
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Connective Phrase
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Location/URL
n/a
ISSN
0043-3144
Notes
n/a
Abstract
Objective: To determine the presence and prevalence of free-living opportunistic protozoan parasites of the genus Acanthamoeba in tap and sea water sources in Jamaica. Method: In this study, 62 samples were collected from beaches and tap water sources across the island of Jamaica. A litre of each was water sample was filtered through a cellulose nitrate filter (0.45 Ám diameter, Millipore Corporation, Bedford, Massachusettes, USA) with a week vacuum (flow rate, 1.3 ml/min). The filters were inverted on 2% Neff's saline non-nutrient agar plates and incubated at 25oC. These plates were monitored for out-growth of Acanthamoeba microscopically and blocks containing Acanthamoeba were removed and the amoebae cloned by dilution. The identification of Acanthamoeba isolates was characterised by temperature, osomotolerance assays and PCR.....
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Keywords