Muzac-Tucker, I; Asemota, Helen N.; Ahmad, Mohammed H.
Department of Basic Medical Sciences; Biotechnology Centre
Analysis of genetic polymorphism in yam DNA by PCR techniques [Abstract]
Greenfield, Madge; Robinson, Ralph
Proceedings of the First Conference of the Faculty of Natural Sciences University of the West Indies, Mona
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University of the West Indies, Mona, Kingston, Jamaica
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Faculty of Natural Sciences, University of the West Indies, Mona.
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The authors research has shown that yam (Dioscorea spp.) tubers grown in Jamaica are bio-chemically diverse (Muzac-Tucker et al. 1993). To establish this at the genetic level, yam DNA was isolated and purified from both tissue cultured plantlets and vines of twelve different cultivars of five common species produced in Jamaica. The DNA was found to be about 49Kb for all yam types. Many restriction endonuclease enzymes were used to characterize the DNA. By the use of PCR (Polymerase Chain Reaction) isolated DNA from various yam species/cultivars were subjected to amplification fingerprinting using various characterized DNA sequences as primers. Results indicated that there is genetic diversity in DNA polymorphism among yam species/cultivars grown in Jamaica.....