Roye, Marcia E. ; McLaughlin, Wayne A. ; Nakhla, Medhat K.; Maxwell, Douglas P.
Biotechnology Centre; Department of Basic Medical Sciences
Genetic diversity and relationships among geminiviruses from Jamaica and Barbados [Abstract]
Fourth Conference of The Faculty of Pure and Applied Sciences. University of the West Indies, Mona.
Date of Meeting
January 12-14, 1999
Place of Meeting
University of the West Indies, Mona. Kingston, Jamaica
Place of Publication
Faculty of Natural Sciences, University of the West Indies, Mona.
Date of Publication
Date of Copyright
Location in Work
Extent of Work
Series Editor Role
Series Volume ID
Geminiviral DNA probes, polymerase chain reaction and nucleotide sequencing was used to identify 7 geminiviruses from Jamaica and 3 from Barbados. PCR-amplified products for DNA-A were generated from 80% of plants from Jamaica that gave hybridisation signals and 32% of plants from Barbados. Nucleotide identity of the common regions of DNA-A and DNA-B, and partial nucleotide sequences of the ac1, av1, bc1, and bv1 genes confirmed the presence of bipartite geminiviruses in the following crops from Jamaica: P. vulgaris and P. lunatus (bean golden mosaic virus, BGMVJM2), L. esculentum and C. chinense (tomato dwarf leaf curl virus, TDLCV), and two distinct viruses infecting cabbage. The weeds associated with viruses includedS ida spp. (sida golden mosaic virus, SiGMV-JM), W. amplissima (wissadula golden mosaic virus, WGMV) and three distinct viruses in M.lathyroides (macroptilium golden mosaic virus strains 1 and 2, MacGMV-JM1, MacGMV-JM2 and SiGMV-JM). Nucleotide sequence analysis of the common region,a c1 and av1 indicate that geminiviral DNA was associated with C. papaya (papaya virus, PaGV). Nucleotide identity of the common regions of DNA-A and DNA-B, and partial nucleotide sequences of the ac1, av1, bc1, and bv1 genes confirmed the same bipartite geminiviruses (from Barbados) in tomato, musk melon and water melon and two distinct geminiviruses iMn lathyroides. Nucleotide sequence alignment of the common region and the 5’ end oafc 1 was used to determine phylogenetic relationships of the new Jamaican viruses with other Western Hemisphere geminiviruses. Geminivirus isolate from P. vulgaris (BGMV-JM1) and an isolate from P. lunatus (BGMV-JM2) was placed in BGMV type II cluster. SiGMV-JM, and MacGMV-JM1, was closely related to potato yellow mosaic geminivirus (PYMV). The viruses associated with W. amplissima (WGMV), C. papaya (PaGV), M. lathyroides (MacGMV-JM2) and tomato and pepper (TDLCV) were closely related to each other. The tomato virus from Barbados was a new previously unreported virus and was distinct from the two macrroptilium viruses. These results indicate that crop-infecting and weed-infecting geminiviruses from Jamaica and Barbados are distinct, highly diverse, and have several geographical origins and weeds are not host to crop-infecting geminiviruses in Jamaica.....