View
Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Naassner, Markus; Mergler, Magnus; Wolf, Klaus W.; Schuphan, Ingolf
Author Affiliation, Ana.
Electron Microscopy Unit
Article Title
Determination of the xenoestrogens 4-nonylphenol and bisphenol A by high-performance liquid chromatography and fluorescence detection after derivatisation with dansyl chloride
Medium Designator
n/a
Connective Phrase
n/a
Journal Title
Journal of Chromatography A
Translated Title
n/a
Reprint Status
n/a
Date of Publication
2002
Volume ID
945
Issue ID
1/2
Page(s)
133-139
Language
n/a
Connective Phrase
n/a
Location/URL
n/a
ISSN
n/a
Notes
n/a
Abstract
An easily performable and highly selective method for the determination of the xenoestrogens bisphenol A (BPA) and technical 4-nonylphenol (mixture of isomers) from environmental samples was developed. The method consists of fluorigenic labelling of the substances by dansylation followed by HPLC separation of the derivatives. Specific wavelengths (lembda(ex)=354 nm, lambda(em)=545 nm) for detection of the dansylated phenols were determined in order to reduce the signals of interfering compounds. The applicablility of the method for environmental samples was demonstrated by using sewage sludge spiked with BPA and 4-n-nonylphenol (as internal standard).....
read more