Lindo, John F.; Conway, David J.; Atkins, Neil S.; Bianco, Albert E.; Robinson, Ralph, D.; Bundy, Donald A. P.
Author Affiliation, Ana.
Microbiology; Basic Medical Sciences (Anatomy, Biochemistry, Physiology)
Prospective evaluation of enzyme-linked immunosorbent assay and immunoblot methods for the diagnosis of endemic Strongyloides stercoralis infection.
American Journal of Tropical Medicine and Hygiene
Date of Publication
Recently described enzyme-linked immunosorbent assay (ELISA) and immunoblot methods for the detection of serum IgG against Strongyloides stercoralis larval antigens were prospectively evaluated for the diagnosis of endemic strongyloidiasis. A modification of the ELISA involved preincubation of sera with Onchocerca gutturosa phosphate-buffered saline-soluble extract to remove cross-reactivity with other helminths. The sensitivity of the ELISA increased from 80 % and 85% following preincubation. Similarly, there was an increase in specificity from 94% to 97%. The IgG recognition of 41-, 31-, and 28-kD filariform larval components showed sensitivities of 100 %, 85% and 65%, respectively. The specificities of recognition of these proteins were 94%, 89% and 75% respectively. Both the ELISA following incubation of sera with O. gutturosa extract and serum IgG reactivity to a 41-kD larval component using immunoblotting are sensitive and specific techniques for diagnosing endemic strongyloidiasis.....