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Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Lindo, John F.; Conway, David J.; Atkins, Neil S.; Bianco, Albert E.; Robinson, Ralph, D.; Bundy, Donald A. P.
Author Affiliation, Ana.
Microbiology; Basic Medical Sciences (Anatomy, Biochemistry, Physiology)
Article Title
Prospective evaluation of enzyme-linked immunosorbent assay and immunoblot methods for the diagnosis of endemic Strongyloides stercoralis infection.
Medium Designator
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Connective Phrase
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Journal Title
American Journal of Tropical Medicine and Hygiene
Translated Title
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Reprint Status
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Date of Publication
1994
Volume ID
51
Issue ID
2
Page(s)
175-9
Language
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Location/URL
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ISSN
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Notes
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Abstract
Recently described enzyme-linked immunosorbent assay (ELISA) and immunoblot methods for the detection of serum IgG against Strongyloides stercoralis larval antigens were prospectively evaluated for the diagnosis of endemic strongyloidiasis. A modification of the ELISA involved preincubation of sera with Onchocerca gutturosa phosphate-buffered saline-soluble extract to remove cross-reactivity with other helminths. The sensitivity of the ELISA increased from 80 % and 85% following preincubation. Similarly, there was an increase in specificity from 94% to 97%. The IgG recognition of 41-, 31-, and 28-kD filariform larval components showed sensitivities of 100 %, 85% and 65%, respectively. The specificities of recognition of these proteins were 94%, 89% and 75% respectively. Both the ELISA following incubation of sera with O. gutturosa extract and serum IgG reactivity to a 41-kD larval component using immunoblotting are sensitive and specific techniques for diagnosing endemic strongyloidiasis.....
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Keywords
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