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Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Peart, Patrice C.; Chen, A.R.M.; Reynolds, W.F.; Reese, Paul B.
Author Affiliation, Ana.
n/a
Article Title
Entrapment of mycelial fragments in calcium alginate: A general technique for the use of immobilized filamentous fungi in biocatalysis
Medium Designator
n/a
Connective Phrase
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Journal Title
Steroids
Translated Title
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Reprint Status
Refereed
Date of Publication
2012
Volume ID
77
Issue ID
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Page(s)
85 - 90
Language
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Connective Phrase
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Location/URL
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ISSN
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Notes
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Abstract
Transformation reactions on 3,17-dihydroxyandrost-5-ene using free fungal cells were compared with those carried out by macerated mycelia, immobilized in calcium alginate beads. Six fungi were utilized in this study, namely Rhizopus oryzae ATCC 11145, Mucor plumbeus ATCC 4740, Cunninghamella echinulata var. elegans ATCC 8688a, Aspergillus niger ATCC 9142, Phanerochaete chrysosporium ATCC 24725 and Whetzelinia sclerotiorum ATCC 18687. The results show, for the first time, that encapsulated mycelial fragments essentially carry out the same bioconversions as those observed with growing cells. As the immobilized cells were 'resting', the products formed were free of contamination by natural products, and this greatly aided the purification of the metabolites. Conditions for bead preparation were optimized. Furthermore, it was noted that the beads could be reused, once they had been subjected to a rejuvenation process.....
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