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Publication Type
Journal Article
UWI Author(s)
Author, Analytic
Stewart, C. S.; Kon, T.; Gilbertson, R. L.; Roye, Marcia E.
Author Affiliation, Ana.
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Article Title
First report of the complete sequence of Sida golden yellow vein virus from Jamaica
Medium Designator
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Connective Phrase
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Journal Title
Archives of Virology
Translated Title
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Reprint Status
Refereed
Date of Publication
2011
Volume ID
156
Issue ID
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Page(s)
1481-1484
Language
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Connective Phrase
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Location/URL
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ISSN
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Notes
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Abstract
Begomoviruses are phytopathogens that threaten food security [18]. Sida spp. are ubiquitous weed species found in Jamaica. Sida samples were collected island-wide, DNA was extracted via a modified Dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. The amplicons were cloned and sequenced. Sequence analysis revealed that a DNA-A molecule isolated from a plant in Liguanea, St. Andrew, was 90.9% similar to Sida golden yellow vein virus-[United States of America:Homestead:A11], making it a strain of SiGYVV. It was named Sida golden yellow vein virus-[Jamaica:Liguanea 2:2008] (SiGYVV-[JM:Lig2:08]). The cognate DNA-B, previously unreported, was successfully cloned and was most similar to that of Malvastrum yellow mosaic Jamaica virus (MaYMJV). Phylogenetic analysis suggested that this virus was most closely related to begomoviruses that infect malvaceous hosts in Jamaica, Cuba and Florida in the United States.....
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Keywords